Application
Dot blot analysis of anti-TSC1-pS505 Phospho-specific Pab (RB13337) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-TSC1-S505 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC1; KIAA0243; TSC; Hamartin; Tuberous sclerosis 1 protein |
| Modification | p-S505 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0243 TSC |
|---|---|
| Alternative Names | TSC1 KIAA0243 TSC Hamartin Tuberous sclerosis 1 protein |
| Molecular Weight(MW) | 129767 Da |
| Function | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling |
| Tissue Specificity | Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes |
| Entrez Gene | 7248 |
|---|
Application
Dot blot analysis of anti-TSC1-pS505 Phospho-specific Pab (RB13337) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
