Application
A. Western blot analysis on HeLa cell lysate using anti-TSC1/Hamartin RabMAb (Cat. #AJ1797a), dilution 1:20,000Application
B. Immunohistochemical analysis of paraffin-embedded human kidneys using anti-TSC-1 RabMAb (Cat. #AJ1797a).| Product Name | TSC1/Hamartin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC1, KIAA0243, TSC, Hamartin, Tuberous sclerosis 1 protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP318Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0243 TSC |
|---|---|
| Alternative Names | TSC1 KIAA0243 TSC Hamartin Tuberous sclerosis 1 protein |
| Molecular Weight(MW) | 129767 Da |
| Function | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling |
| Tissue Specificity | Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes |
| Entrez Gene | 7248 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-TSC1/Hamartin RabMAb (Cat. #AJ1797a), dilution 1:20,000
Application
B. Immunohistochemical analysis of paraffin-embedded human kidneys using anti-TSC-1 RabMAb (Cat. #AJ1797a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
