Application
Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-a (20ng/ml, 5min), using 14-3-3 Zeta (Delta) Antibody (S58) (#AE1001a, lane 1 and 2) and Phospho-14-3-3 Zeta (Delta)-S58 Antibody (AE1001b, lane 3 and 4).Application
Immunohistochemical analysis of paraffin-embeddedhuman breast carcinoma tissue, using Phospho-14-3-3 Zeta (Delta)-S58 Antibody (#AE1001b).| Product Name | Phospho-YWHAZ-pS58 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase C inhibitor protein 1, KCIP-1; 14-3-3 protein zeta/delta |
| Modification | p-S58 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Protein kinase C inhibitor protein 1 KCIP-1 14-3-3 protein zeta/delta |
|---|---|
| Molecular Weight(MW) | 27745 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes |
| Entrez Gene | 7534 |
|---|
Application
Western blot analysis of extract from NIH/3T3 cells, untreated or treated with TNF-a (20ng/ml, 5min), using 14-3-3 Zeta (Delta) Antibody (S58) (#AE1001a, lane 1 and 2) and Phospho-14-3-3 Zeta (Delta)-S58 Antibody (AE1001b, lane 3 and 4).
Application
Immunohistochemical analysis of paraffin-embeddedhuman breast carcinoma tissue, using Phospho-14-3-3 Zeta (Delta)-S58 Antibody (#AE1001b).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
