Application
Western blot analysis of PHTF1 Antibody (Center) in Hela cell line lysates (35ug/lane). PHTF1 (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human testis tissue reacted with PHTF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of PHTF1 Antibody (Center) with paraffin-embedded human testis tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Application
PHTF1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PHTF1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHTF1; PHTF; Putative homeodomain transcription factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PHTF |
|---|---|
| Alternative Names | PHTF1 PHTF Putative homeodomain transcription factor 1 |
| Molecular Weight(MW) | 87252 Da |
| Function | May play a role in transcription regulation. |
| Tissue Specificity | Widely expressed with highest levels in testis |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 10745 |
|---|
Application
Western blot analysis of PHTF1 Antibody (Center) in Hela cell line lysates (35ug/lane). PHTF1 (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human testis tissue reacted with PHTF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of PHTF1 Antibody (Center) with paraffin-embedded human testis tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Application
PHTF1 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
