Application
A. Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a), dilution 1:2,000. A: A431 B: MCF-7 C: RajiApplication
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a).Application
C. Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a).| Product Name | PI3-kinase (p85) Antibody (subunit alpha) (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase p85 subunit alpha;PtdIns-3-kinase p85-alpha |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP380Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRB1 |
|---|---|
| Alternative Names | PIK3R1 GRB1 Phosphatidylinositol 3-kinase regulatory subunit alpha PI3-kinase p85 subunit alpha PtdIns-3-kinase p85-alpha |
| Molecular Weight(MW) | 83598 Da |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling |
| Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level) |
| Entrez Gene | 5295 |
|---|
Application
A. Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a), dilution 1:2,000. A: A431 B: MCF-7 C: Raji
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a).
Application
C. Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha RabMAb (Cat. #AJ1608a).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
