Application
Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution)Application
Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PI3KCA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIK3CA; Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PIK3CA Phosphatidylinositol 4 5-bisphosphate 3-kinase catalytic subunit alpha isoform Phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha Phosphoinositide-3-kinase catalytic alpha polypeptide Serine/threonine protein kinase |
|---|---|
| Molecular Weight(MW) | 124284 Da |
| Function | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS |
| Tissue Specificity | This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511~541 amino acids from the the center region of human PI3KCA. |
Application
Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution)
Application
Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
