Application
A. Western blot analysis on (A) Daudi (B) 293 (C) Jurkat cell lysate, dilution 1:50,000 and (D) SW480 and (E) HepG2 using anti-PIAS1 RabMAb (Cat. #AJ1609a)Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-PIAS1 RabMAb (Cat. #AJ1609a).| Product Name | PIAS1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIAS1, DDXBP1, E3 SUMO-protein ligase PIAS1, RNA helicase II-binding protein;DEAD/H box-binding protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2581Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1: 1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDXBP1 |
|---|---|
| Alternative Names | PIAS1 DDXBP1 E3 SUMO-protein ligase PIAS1 RNA helicase II-binding protein DEAD/H box-binding protein 1 |
| Molecular Weight(MW) | 71836 Da |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling |
| Tissue Specificity | Expressed in numerous tissues with highest level in testis |
| Cellular Localization | Nucleus speckle. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton |
| Entrez Gene | 8554 |
|---|
Application
A. Western blot analysis on (A) Daudi (B) 293 (C) Jurkat cell lysate, dilution 1:50,000 and (D) SW480 and (E) HepG2 using anti-PIAS1 RabMAb (Cat. #AJ1609a)
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-PIAS1 RabMAb (Cat. #AJ1609a).| Application Notes | WB:1: 1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
