Application
The PIAS1 polyclonal antibody is used in Western blot to detect PIAS1 in HL-60 tissue lysate.Application
Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PIAS1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIAS1; DDXBP1; E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDXBP1 |
|---|---|
| Alternative Names | PIAS1 DDXBP1 E3 SUMO-protein ligase PIAS1 DEAD/H box-binding protein 1 Gu-binding protein Protein inhibitor of activated STAT protein 1 RNA helicase II-binding protein |
| Molecular Weight(MW) | 71836 Da |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling |
| Tissue Specificity | Expressed in numerous tissues with highest level in testis |
| Cellular Localization | Nucleus speckle. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton |
| Entrez Gene | 8554 |
|---|
Application
The PIAS1 polyclonal antibody is used in Western blot to detect PIAS1 in HL-60 tissue lysate.
Application
Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
