Application
PIAS2 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIAS2 antibody detected the PIAS2 protein (arrow).Application
PIAS2 Antibody (N-term) (AP13993a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIAS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PIAS2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIAS2; PIASX; E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; DAB2-interacting protein; Msx-interacting zinc finger protein; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PIASX |
|---|---|
| Alternative Names | PIAS2 PIASX E3 SUMO-protein ligase PIAS2 Androgen receptor-interacting protein 3 DAB2-interacting protein Msx-interacting zinc finger protein PIAS-NY protein Protein inhibitor of activated STAT x Protein inhibitor of activated STAT2 |
| Molecular Weight(MW) | 68240 Da |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha |
| Tissue Specificity | Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs |
| Cellular Localization | Nucleus speckle. Nucleus, PML body. Note=Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) |
| Entrez Gene | 9063 |
|---|---|
| Nucleotide Accession | NP_004662.2 NP_775298.1 |
Application
PIAS2 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIAS2 antibody detected the PIAS2 protein (arrow).
Application
PIAS2 Antibody (N-term) (AP13993a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIAS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
