Application
Western blot analysis of PIGM Antibody (C-term) in T47D cell line lysates (35ug/lane). PIGM (arrow) was detected using the purified Pab.Application
PIGM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PIGM Antibody (C-term) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PIGM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIGM; GPI mannosyltransferase 1; GPI mannosyltransferase I; Phosphatidylinositol-glycan biosynthesis class M protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PIGM GPI mannosyltransferase 1 GPI mannosyltransferase I Phosphatidylinositol-glycan biosynthesis class M protein |
|---|---|
| Molecular Weight(MW) | 49460 Da |
| Function | Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly |
| Tissue Specificity | This PIGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394~423 amino acids from the C-terminal region of human PIGM. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 93183 |
|---|
Application
Western blot analysis of PIGM Antibody (C-term) in T47D cell line lysates (35ug/lane). PIGM (arrow) was detected using the purified Pab.
Application
PIGM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PIGM Antibody (C-term) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
