Application
Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PIGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PIGR Antibody (C-term) (Cat#AP8555b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of HepG2 cells using PIGR Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PIGR Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIGR; Polymeric immunoglobulin receptor; Hepatocellular carcinoma-associated protein TB6; Secretory component |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PIGR Polymeric immunoglobulin receptor Hepatocellular carcinoma-associated protein TB6 Secretory component |
|---|---|
| Molecular Weight(MW) | 83284 Da |
| Function | This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment |
| Tissue Specificity | This PIGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 652~681 amino acids from the C-terminal region of human PIGR. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 5284 |
|---|
Application
Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PIGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PIGR Antibody (C-term) (Cat#AP8555b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of HepG2 cells using PIGR Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
