Application
PIGZ Antibody (C-term) western blot analysis in CEM cell line lysates (15ug/lane).This demonstrates the PIGZ antibody detected the PIGZ protein (arrow).Application
PIGZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PIGZ Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PIGZ Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIGZ; SMP3; GPI mannosyltransferase 4; GPI mannosyltransferase IV; Phosphatidylinositol-glycan biosynthesis class Z protein; SMP3 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMP3 |
|---|---|
| Alternative Names | PIGZ SMP3 GPI mannosyltransferase 4 GPI mannosyltransferase IV Phosphatidylinositol-glycan biosynthesis class Z protein SMP3 homolog |
| Molecular Weight(MW) | 63471 Da |
| Function | Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues |
| Tissue Specificity | Widely expressed at low level, with highest level in brain and colon |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 80235 |
|---|---|
| Nucleotide Accession | NP_079439.2 |
Application
PIGZ Antibody (C-term) western blot analysis in CEM cell line lysates (15ug/lane).This demonstrates the PIGZ antibody detected the PIGZ protein (arrow).
Application
PIGZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PIGZ Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
