Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Pin1 (pS16) RabMAb (Cat. #AJ1611b), 1:500 dilution. Cells were either (A) untreated or (B) treated with FBS + Calyculin A.| Product Name | Pin1 Antibody Phospho (pS16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Rotamase Pin1;PPIase Pin1 |
| Modification | p-S16 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1480Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PIN1 Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 PPIase Pin1 |
|---|---|
| Molecular Weight(MW) | 18243 Da |
| Function | Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation |
| Tissue Specificity | The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells |
| Cellular Localization | Nucleus. Nucleus speckle. Cytoplasm. Note=Co-localizes with NEK6 in the nucleus. Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization |
| Entrez Gene | 5300 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Pin1 (pS16) RabMAb (Cat. #AJ1611b), 1:500 dilution. Cells were either (A) untreated or (B) treated with FBS + Calyculin A.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
