Application
Western blot analysis of PINX1 Antibody (Center) in A375 cell line lysates (35ug/lane). PINX1 (arrow) was detected using the purified Pab.Application
PINX1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PINX1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PINX1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PINX1; LPTL; LPTS; PIN2/TERF1-interacting telomerase inhibitor 1; Liver-related putative tumor suppressor; Pin2-interacting protein X1; Protein 67-11-3; TRF1-interacting protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LPTL LPTS |
|---|---|
| Alternative Names | PINX1 LPTL LPTS PIN2/TERF1-interacting telomerase inhibitor 1 Liver-related putative tumor suppressor Pin2-interacting protein X1 Protein 67-11-3 TRF1-interacting protein 1 |
| Molecular Weight(MW) | 37035 Da |
| Function | Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor |
| Tissue Specificity | Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Note=Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE |
| Entrez Gene | 54984 |
|---|
Application
Western blot analysis of PINX1 Antibody (Center) in A375 cell line lysates (35ug/lane). PINX1 (arrow) was detected using the purified Pab.
Application
PINX1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PINX1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
