Application
"Western blot analysis of PIP5K3 (arrow) using rabbit polyclonal PIP5K3 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2) (Origene Technologies)."Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PIP5K3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIKFYVE; KIAA0981; PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0981 PIP5K3 |
|---|---|
| Alternative Names | PIKFYVE KIAA0981 PIP5K3 1-phosphatidylinositol 3-phosphate 5-kinase FYVE finger-containing phosphoinositide kinase PIKfyve Phosphatidylinositol 3-phosphate 5-kinase type III |
| Molecular Weight(MW) | 237136 Da |
| Function | The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate Required for endocytic-vacuolar pathway and nuclear migration Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes |
| Tissue Specificity | This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the center region of human PIP5K3. |
| Cellular Localization | Endosome membrane. Note=Mainly associated with membranes of the late endocytic pathway |
| Entrez Gene | 200576 |
|---|
Application
"Western blot analysis of PIP5K3 (arrow) using rabbit polyclonal PIP5K3 Antibody (Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2) (Origene Technologies)."
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
