Application
Western blot analysis of hNCOA6IP N-term D5 in NIH-3T3 cell line lysates (35ug/lane). NCOA6IP (arrow) was detected using the purified Pab.| Product Name | PIPMT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TGS1; HCA137; NCOA6IP; PIMT; Trimethylguanosine synthase; CLL-associated antigen KW-2; Cap-specific guanine-N2 methyltransferase; Hepatocellular carcinoma-associated antigen 137; Nuclear receptor coactivator 6-interacting protein; PRIP-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HCA137 NCOA6IP PIMT |
|---|---|
| Alternative Names | TGS1 HCA137 NCOA6IP PIMT Trimethylguanosine synthase CLL-associated antigen KW-2 Cap-specific guanine-N2 methyltransferase Hepatocellular carcinoma-associated antigen 137 Nuclear receptor coactivator 6-interacting protein PRIP-interacting protein |
| Molecular Weight(MW) | 96620 Da |
| Function | Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation |
| Tissue Specificity | Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta |
| Cellular Localization | Cytoplasm. Nucleus, Cajal body. Note=A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network |
| Entrez Gene | 96764 |
|---|
Application
Western blot analysis of hNCOA6IP N-term D5 in NIH-3T3 cell line lysates (35ug/lane). NCOA6IP (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
