Application
Western blot analysis of PIR antibody (Center) (Cat.# AP7619c) in mouse liver tissue lysates (35ug/lane). PIR (arrow) was detected using the purified Pab.Application
PIR Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PIR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIR; Pirin; Probable quercetin 2, 3-dioxygenase PIR |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PIR Pirin Probable quercetin 2 3-dioxygenase PIR |
|---|---|
| Molecular Weight(MW) | 32113 Da |
| Function | Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro) |
| Tissue Specificity | Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level) Expressed in all tissues, with highest level of expression in heart and liver |
| Cellular Localization | Nucleus. Cytoplasm. Note=Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression |
| Entrez Gene | 8544 |
|---|
Application
Western blot analysis of PIR antibody (Center) (Cat.# AP7619c) in mouse liver tissue lysates (35ug/lane). PIR (arrow) was detected using the purified Pab.
Application
PIR Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
