Application
PJA1 Antibody (W371) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).Application
PJA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PJA Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PJA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PJA1; RNF70; E3 ubiquitin-protein ligase Praja-1; RING finger protein 70 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF70 |
|---|---|
| Alternative Names | PJA1 RNF70 E3 ubiquitin-protein ligase Praja-1 RING finger protein 70 |
| Molecular Weight(MW) | 71002 Da |
| Function | Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting |
| Tissue Specificity | Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex |
| Entrez Gene | 64219 |
|---|
Application
PJA1 Antibody (W371) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).
Application
PJA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PJA Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.10% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
