Application
A. Western blot analysis on (A) Fetal brain, (B) Human testis and (C) NIH/3T3 cell lysates using anti-PKA, RIIb RabMAb (Cat. #AJ1615a), dilution 1:20,000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PKA, RIIb RabMAb (Cat. #AJ1615a).| Product Name | PKA, RIIb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAR2B, cAMP-dependent protein kinase type II-beta regulatory subunit |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2648 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRKAR2B cAMP-dependent protein kinase type II-beta regulatory subunit |
|---|---|
| Molecular Weight(MW) | 46302 Da |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase |
| Tissue Specificity | Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and at the cell membrane |
| Entrez Gene | 5577 |
|---|
Application
A. Western blot analysis on (A) Fetal brain, (B) Human testis and (C) NIH/3T3 cell lysates using anti-PKA, RIIb RabMAb (Cat. #AJ1615a), dilution 1:20,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PKA, RIIb RabMAb (Cat. #AJ1615a).| Application Notes | WB:1:5000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
