Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PKN beta Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKN3; PKNBETA; Serine/threonine-protein kinase N3; Protein kinase PKN-beta; Protein-kinase C-related kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKNBETA |
|---|---|
| Alternative Names | PKN3 PKNBETA Serine/threonine-protein kinase N3 Protein kinase PKN-beta Protein-kinase C-related kinase 3 |
| Molecular Weight(MW) | 99421 Da |
| Function | Contributes to invasiveness in malignant prostate cancer |
| Tissue Specificity | Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues |
| Cellular Localization | Nucleus. Cytoplasm, perinuclear region. Note=Nuclear and perinuclear Golgi region |
Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
