Application
A. Western blot analysis on HeLa cell lysates using anti-phosho PKR (pT451) RabMAb (Cat. #AJ1627d), 1:2,000 dilution. Cells were either (A) untreated (B) treated with IFN-alpha and Calyculin A.| Product Name | PKR Antibody Phospho (pT451) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2AK2, PKR, PRKR, Interferon-induced, double-stranded RNA-activated protein kinase, p68 kinase;P1/eIF-2A protein kinase |
| Modification | p-T451 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2152Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKR PRKR |
|---|---|
| Alternative Names | EIF2AK2 PKR PRKR Interferon-induced double-stranded RNA-activated protein kinase p68 kinase P1/eIF-2A protein kinase |
| Molecular Weight(MW) | 62094 Da |
| Function | Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity: phosphorylates CDK1 upon DNA damage. CDK1 phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr451 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. The antibody only detects PKR phosphorylated on Threonine 451. |
| Entrez Gene | 5610 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-phosho PKR (pT451) RabMAb (Cat. #AJ1627d), 1:2,000 dilution. Cells were either (A) untreated (B) treated with IFN-alpha and Calyculin A.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
