Application
A. Western blot analysis on Raji cell lysates using anti-Phospho- PLC?II (pY1217) RabMAb (Cat. #AJ1629b). Cells were either (A) untreated (B) treated with pervanadate.Application
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Phospho- PLC?II (pY1217) RabMAb (Cat. #AJ1629b).| Product Name | PLC II Antibody Phospho (pY1217) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCG2, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV |
| Modification | p-Y1217 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1404Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PLCG2 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-2 PLC-IV |
|---|---|
| Molecular Weight(MW) | 147870 Da |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Tyr1217 of human PLCγII was used as immunogen. The antibody only detects PLCγII phosphorylated onTyrosine 1217. |
| Entrez Gene | 5336 |
|---|
Application
A. Western blot analysis on Raji cell lysates using anti-Phospho- PLC?II (pY1217) RabMAb (Cat. #AJ1629b). Cells were either (A) untreated (B) treated with pervanadate.
Application
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Phospho- PLC?II (pY1217) RabMAb (Cat. #AJ1629b).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
