Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PLCB3 (pS537) RabMAb (Cat. #AJ1633a), 1:2000 dilution. Cells were either (A) untreated or (B) treated with TPA.| Product Name | PLC-III Antibody Phospho (pS537) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCB3, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-3, Phosphoinositide phospholipase C;Phospholipase C-beta-3 |
| Modification | p-S537 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1498Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PLCB3 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase beta-3 Phosphoinositide phospholipase C Phospholipase C-beta-3 |
|---|---|
| Molecular Weight(MW) | 138799 Da |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes |
| Tissue Specificity | A phosphor-specific peptide corresponding to residues surrounding Serine 537 of human PLC-β3 was used as an immunogen. This antibody detects PLC- β3 phosphorylated on Ser537. |
| Cellular Localization | Membrane; Peripheral membrane protein. |
| Entrez Gene | 5331 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PLCB3 (pS537) RabMAb (Cat. #AJ1633a), 1:2000 dilution. Cells were either (A) untreated or (B) treated with TPA.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
