Application
PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).Application
PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PLCL2 Antibody (C-term)(Cat#AP12004b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PLCL2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCL2; KIAA1092; PLCE2; Inactive phospholipase C-like protein 2; Phospholipase C-epsilon-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1092 PLCE2 |
|---|---|
| Alternative Names | PLCL2 KIAA1092 PLCE2 Inactive phospholipase C-like protein 2 Phospholipase C-epsilon-2 |
| Molecular Weight(MW) | 125866 Da |
| Function | May play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity) |
| Tissue Specificity | This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1100-1127 amino acids from the C-terminal region of human PLCL2. |
| Cellular Localization | Cytoplasm. Note=Predominantly localized to perinuclear areas in both myoblast and myotube C2C12 cells (By similarity) |
| Entrez Gene | 23228 |
|---|---|
| Nucleotide Accession | NP_055999.2 |
Application
PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).
Application
PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PLCL2 Antibody (C-term)(Cat#AP12004b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
