Application
PLD6 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).| Product Name | PLD6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLD6; Mitochondrial cardiolipin hydrolase; Choline phosphatase 6; Mitochondrial phospholipase; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D6; Protein zucchini homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PLD6 Mitochondrial cardiolipin hydrolase Choline phosphatase 6 Mitochondrial phospholipase Phosphatidylcholine-hydrolyzing phospholipase D6 Phospholipase D6 Protein zucchini homolog |
|---|---|
| Molecular Weight(MW) | 28273 Da |
| Function | Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons |
| Tissue Specificity | Widely expressed. Expression is not limites to testis and ovary |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein |
| Entrez Gene | 201164 |
|---|---|
| Nucleotide Accession | NP_849158.1 |
Application
PLD6 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
