Application
Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.| Product Name | PLCZ1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCZ1; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase zeta-1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C-zeta-1; Testis-development protein NYD-SP27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PLCZ1 1-phosphatidylinositol 4 5-bisphosphate phosphodiesterase zeta-1 Phosphoinositide phospholipase C-zeta-1 Phospholipase C-zeta-1 Testis-development protein NYD-SP27 |
|---|---|
| Molecular Weight(MW) | 70411 Da |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function |
| Tissue Specificity | Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic- duct cells from patients with cystic fibrosis |
| Cellular Localization | Nucleus (By similarity). Cytoplasm, perinuclear region (By similarity). Note=Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity) |
| Entrez Gene | 89869 |
|---|
Application
Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
