Application
Western blot analysis of PLD1 (arrow) using rabbit polyclonal PLD1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLD1 gene.Application
PLD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PLD1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PLD1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLD1; Phospholipase D1; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PLD1 Phospholipase D1 Choline phosphatase 1 Phosphatidylcholine-hydrolyzing phospholipase D1 |
|---|---|
| Molecular Weight(MW) | 124184 Da |
| Function | Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity) |
| Tissue Specificity | Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine |
| Cellular Localization | Cytoplasm, perinuclear region (By similarity). Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side (By similarity). Golgi apparatus membrane; Lipid- anchor; Cytoplasmic side (By similarity). Late endosome membrane; Lipid-anchor; Cytoplasmic side (By similarity) |
| Entrez Gene | 5337 |
|---|---|
| Nucleotide Accession | NP_001123553.1 NP_002653.1 |
Application
Western blot analysis of PLD1 (arrow) using rabbit polyclonal PLD1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLD1 gene.
Application
PLD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PLD1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
