Application
The anti-SNK C-term Pab is used in Western blot to detect SNK in PMA-treated Pam212 cell lysate (lane 1) and rat testis tissue lysate (lane 2).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SNK Antibody (C-term ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PLK2 (SNK) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLK2; SNK; Serine/threonine-protein kinase PLK2; Polo-like kinase 2; Serine/threonine-protein kinase SNK; Serum-inducible kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SNK |
|---|---|
| Alternative Names | PLK2 SNK Serine/threonine-protein kinase PLK2 Polo-like kinase 2 Serine/threonine-protein kinase SNK Serum-inducible kinase |
| Molecular Weight(MW) | 78237 Da |
| Function | Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress |
| Tissue Specificity | Expressed at higher level in the fetal lung, kidney, spleen and heart |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome, centriole. Cell projection, dendrite (By similarity) Note=Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase |
| Entrez Gene | 10769 |
|---|
Application
The anti-SNK C-term Pab is used in Western blot to detect SNK in PMA-treated Pam212 cell lysate (lane 1) and rat testis tissue lysate (lane 2).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SNK Antibody (C-term ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
