Application
PLOD1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow).Application
PLOD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PLOD1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PLOD1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLOD1; LLH; PLOD; Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LLH PLOD |
|---|---|
| Alternative Names | PLOD1 LLH PLOD Procollagen-lysine 2-oxoglutarate 5-dioxygenase 1 Lysyl hydroxylase 1 |
| Molecular Weight(MW) | 83550 Da |
| Function | Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links |
| Tissue Specificity | This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human PLOD1. |
| Cellular Localization | Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side |
| Entrez Gene | 5351 |
|---|---|
| Nucleotide Accession | NP_000293.2 |
Application
PLOD1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow).
Application
PLOD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PLOD1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
