Application
PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).Application
PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).Application
PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.| Product Name | PMCH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PMCH; MCH; Pro-MCH; Neuropeptide-glycine-glutamic acid; Neuropeptide-glutamic acid-isoleucine; Melanin-concentrating hormone |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH |
|---|---|
| Alternative Names | PMCH MCH Pro-MCH Neuropeptide-glycine-glutamic acid Neuropeptide-glutamic acid-isoleucine Melanin-concentrating hormone |
| Molecular Weight(MW) | 18679 Da |
| Function | MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation |
| Tissue Specificity | Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood. In brain exclusively mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly |
| Cellular Localization | Secreted. |
| Entrez Gene | 5367 |
|---|---|
| Nucleotide Accession | NP_002665.2 |
Application
PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).
Application
PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).
Application
PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
