Application
"Western blot analysis of PMAT(Slc29a4) (arrow) using rabbit polyclonal PMAT(Slc29a4) Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PMAT(Slc29a4) gene (Lane 2) (Origene Technologies)."| Product Name | PMAT(Slc29a4) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Slc29a4; Ent4; Equilibrative nucleoside transporter 4; Solute carrier family 29 member 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Ent4 |
|---|---|
| Alternative Names | Slc29a4 Ent4 Equilibrative nucleoside transporter 4 Solute carrier family 29 member 4 |
| Molecular Weight(MW) | 58099 Da |
| Function | Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorptprev reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In (PubMed:16873718) adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia (By similarity) |
| Tissue Specificity | Expressed in heart. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein (Potential). Apical cell membrane; Multi-pass membrane protein (By similarity). Note=Located to the plasma membranes of ventricular myocytes and vascular endothelial cells. Targeted to the apical membranes of differentiated kidney epithelial cells (By similarity) |
| Entrez Gene | 243328 |
|---|
Application
"Western blot analysis of PMAT(Slc29a4) (arrow) using rabbit polyclonal PMAT(Slc29a4) Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PMAT(Slc29a4) gene (Lane 2) (Origene Technologies)."| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
