Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.| Product Name | PNCK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PNCK; Calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PNCK Calcium/calmodulin-dependent protein kinase type 1B CaM kinase I beta Pregnancy up-regulated non-ubiquitously-expressed CaM kinase |
|---|---|
| Molecular Weight(MW) | 38500 Da |
| Function | Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity) |
| Tissue Specificity | This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314~343 amino acids from the C-terminal region of human PNCK. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 139728 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
