Application
PNKP Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PNKP antibody detected the PNKP protein (arrow).Application
Western blot analysis of PNKP (arrow) using rabbit polyclonal PNKP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.| Product Name | PNKP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PNKP; Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; Polynucleotide 3'-phosphatase; 2'(3')-polynucleotidase; Polynucleotide 5'-hydroxyl-kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PNKP Bifunctional polynucleotide phosphatase/kinase DNA 5'-kinase/3'-phosphatase Polynucleotide kinase-3'-phosphatase Polynucleotide 3'-phosphatase 2'(3')-polynucleotidase Polynucleotide 5'-hydroxyl-kinase |
|---|---|
| Molecular Weight(MW) | 57076 Da |
| Function | Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone |
| Tissue Specificity | Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193) |
| Cellular Localization | Nucleus. |
| Entrez Gene | 11284 |
|---|---|
| Nucleotide Accession | NP_009185.2 |
Application
PNKP Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PNKP antibody detected the PNKP protein (arrow).
Application
Western blot analysis of PNKP (arrow) using rabbit polyclonal PNKP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
