Application
PNPT1 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).Application
PNPT1 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).| Product Name | PNPT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PNPT1; PNPASE; Polyribonucleotide nucleotidyltransferase 1, mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PNPASE |
|---|---|
| Alternative Names | PNPT1 PNPASE Polyribonucleotide nucleotidyltransferase 1 mitochondrial 3'-5' RNA exonuclease OLD35 PNPase old-35 Polynucleotide phosphorylase 1 Polynucleotide phosphorylase-like protein |
| Molecular Weight(MW) | 85951 Da |
| Function | RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c- myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA |
| Tissue Specificity | This PNPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the Central region of human PNPT1. |
| Cellular Localization | Cytoplasm. Mitochondrion. Mitochondrion intermembrane space; Peripheral membrane protein |
| Entrez Gene | 87178 |
|---|---|
| Nucleotide Accession | NP_149100.2 |
Application
PNPT1 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).
Application
PNPT1 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PNPT1 antibody detected the PNPT1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
