Application
POFUT1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT1 antibody detected the POFUT1 protein (arrow).Application
POFUT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POFUT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POFUT1; FUT12; KIAA0180; GDP-fucose protein O-fucosyltransferase 1; Peptide-O-fucosyltransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FUT12 KIAA0180 |
|---|---|
| Alternative Names | POFUT1 FUT12 KIAA0180 GDP-fucose protein O-fucosyltransferase 1 Peptide-O-fucosyltransferase 1 |
| Molecular Weight(MW) | 43956 Da |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Endoplasmic reticulum (By similarity). |
| Entrez Gene | 23509 |
|---|---|
| Nucleotide Accession | NP_056167.1 |
Application
POFUT1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT1 antibody detected the POFUT1 protein (arrow).
Application
POFUT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
