Application
POFUT2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).Application
POFUT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POFUT2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POFUT2; C21orf80; FUT13; KIAA0958; GDP-fucose protein O-fucosyltransferase 2; Peptide-O-fucosyltransferase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C21orf80 FUT13 KIAA0958 |
|---|---|
| Alternative Names | POFUT2 C21orf80 FUT13 KIAA0958 GDP-fucose protein O-fucosyltransferase 2 Peptide-O-fucosyltransferase 2 |
| Molecular Weight(MW) | 49976 Da |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 |
| Tissue Specificity | Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart |
| Cellular Localization | Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum |
| Entrez Gene | 23275 |
|---|---|
| Nucleotide Accession | NP_598368.2 |
Application
POFUT2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).
Application
POFUT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
