Application
POLG Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).Application
POLG Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of POLG Antibody (C-term)(Cat#AP14948b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POLG Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLG; MDP1; POLG1; POLGA; DNA polymerase subunit gamma-1; Mitochondrial DNA polymerase catalytic subunit; PolG-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MDP1 POLG1 POLGA |
|---|---|
| Alternative Names | POLG MDP1 POLG1 POLGA DNA polymerase subunit gamma-1 Mitochondrial DNA polymerase catalytic subunit PolG-alpha |
| Molecular Weight(MW) | 139562 Da |
| Function | Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA |
| Tissue Specificity | This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1119-1148 amino acids from the C-terminal region of human POLG. |
| Cellular Localization | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid |
| Entrez Gene | 5428 |
|---|---|
| Nucleotide Accession | NP_001119603.1 NP_002684.1 |
Application
POLG Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).
Application
POLG Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of POLG Antibody (C-term)(Cat#AP14948b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
POLG Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
