Application
Western blot analysis of POLD1 Antibody (N-term) in A2058 and A375 cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.Application
POLD1 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | POLD1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLD1; POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | POLD |
|---|---|
| Alternative Names | POLD1 POLD DNA polymerase delta catalytic subunit DNA polymerase subunit delta p125 |
| Molecular Weight(MW) | 123631 Da |
| Function | Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex |
| Tissue Specificity | This POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~47 amino acids from the N-term region of human POLD1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5424 |
|---|
Application
Western blot analysis of POLD1 Antibody (N-term) in A2058 and A375 cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.
Application
POLD1 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
