Application
POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).Application
POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | POLR1B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR1B; DNA-directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135 kDa polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR1B DNA-directed RNA polymerase I subunit RPA2 DNA-directed RNA polymerase I 135 kDa polypeptide |
|---|---|
| Molecular Weight(MW) | 128229 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity) |
| Tissue Specificity | This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the N-terminal region of human POLR1B. |
| Cellular Localization | Nucleus, nucleolus. |
| Entrez Gene | 84172 |
|---|---|
| Nucleotide Accession | NP_061887.2 NP_001131076.1 |
Application
POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).
Application
POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
