Application
POLI Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POLI antibody detected the POLI protein (arrow).Application
POLI Antibody (C-term) (AP14526b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLI Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POLI Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAD30B |
|---|---|
| Alternative Names | POLI RAD30B DNA polymerase iota Eta2 RAD30 homolog B |
| Molecular Weight(MW) | 83006 Da |
| Function | Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity |
| Tissue Specificity | Ubiquitous. Highly expressed in testis. |
| Cellular Localization | Nucleus. Note=Accumulates at replication forks after DNA damage |
| Entrez Gene | 11201 |
|---|---|
| Nucleotide Accession | NP_009126.2 |
Application
POLI Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POLI antibody detected the POLI protein (arrow).
Application
POLI Antibody (C-term) (AP14526b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLI Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
