Application
POLK Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLK antibody detected the POLK protein (arrow).| Product Name | POLK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLK; DINB1; DNA polymerase kappa; DINB protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DINB1 |
|---|---|
| Alternative Names | POLK DINB1 DNA polymerase kappa DINB protein |
| Molecular Weight(MW) | 98809 Da |
| Function | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity |
| Tissue Specificity | Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes |
| Cellular Localization | Nucleus. Note=Detected throughout the nucleus and at replication foci |
| Entrez Gene | 51426 |
|---|---|
| Nucleotide Accession | NP_057302.1 |
Application
POLK Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLK antibody detected the POLK protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
