Application
Western blot analysis of POLR1D Antibody (C-term) in HL-60 cell line lysates (35ug/lane). POLR1D (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with POLR1D Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
POLR1D Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | POLR1D Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR1D; DNA-directed RNA polymerases I and III subunit RPAC2; AC19; DNA-directed RNA polymerase I subunit D; RNA polymerase I 16 kDa subunit; RPC16; hRPA19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR1D DNA-directed RNA polymerases I and III subunit RPAC2 AC19 DNA-directed RNA polymerase I subunit D RNA polymerase I 16 kDa subunit RPC16 hRPA19 |
|---|---|
| Molecular Weight(MW) | 15237 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively |
| Tissue Specificity | This POLR1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104~133 amino acids from the C-terminal region of human POLR1D. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 51082 |
|---|
Application
Western blot analysis of POLR1D Antibody (C-term) in HL-60 cell line lysates (35ug/lane). POLR1D (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with POLR1D Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
POLR1D Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
