Application
POLR1A Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the POLR1A antibody detected the POLR1A protein (arrow).| Product Name | POLR1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR1A; DNA-directed RNA polymerase I subunit RPA1; A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR1A DNA-directed RNA polymerase I subunit RPA1 A190 DNA-directed RNA polymerase I largest subunit DNA-directed RNA polymerase I subunit A RNA polymerase I 194 kDa subunit |
|---|---|
| Molecular Weight(MW) | 194811 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity) |
| Tissue Specificity | This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A. |
| Cellular Localization | Nucleus, nucleolus (By similarity). |
| Entrez Gene | 25885 |
|---|---|
| Nucleotide Accession | NP_056240.2 |
Application
POLR1A Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the POLR1A antibody detected the POLR1A protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
