Application
POLR2D Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POLR2D antibody detected the POLR2D protein (arrow).Application
Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.| Product Name | POLR2D Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2D; DNA-directed RNA polymerase II subunit RPB4; DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR2D DNA-directed RNA polymerase II subunit RPB4 DNA-directed RNA polymerase II subunit D RNA polymerase II 16 kDa subunit |
|---|---|
| Molecular Weight(MW) | 16311 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity) |
| Tissue Specificity | This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-79 amino acids from the Central region of human POLR2D. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5433 |
|---|---|
| Nucleotide Accession | NP_004796.1 |
Application
POLR2D Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POLR2D antibody detected the POLR2D protein (arrow).
Application
Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
