Application
POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).| Product Name | POLR2E Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2E; DNA-directed RNA polymerases I, II, and III subunit RPABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR2E DNA-directed RNA polymerases I II and III subunit RPABC1 DNA-directed RNA polymerase II 23 kDa polypeptide DNA-directed RNA polymerase II subunit E RPB5 homolog XAP4 |
|---|---|
| Molecular Weight(MW) | 24551 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity) |
| Tissue Specificity | This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of human POLR2E. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5434 |
|---|---|
| Nucleotide Accession | NP_002686.2 |
Application
POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
