Application
POLR2B Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2B antibody detected the POLR2B protein (arrow).| Product Name | POLR2B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2B; DNA-directed RNA polymerase II subunit RPB2; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR2B DNA-directed RNA polymerase II subunit RPB2 DNA-directed RNA polymerase II 140 kDa polypeptide DNA-directed RNA polymerase II subunit B RNA polymerase II subunit 2 RNA polymerase II subunit B2 |
|---|---|
| Molecular Weight(MW) | 133897 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity) |
| Tissue Specificity | This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-826 amino acids from the Central region of human POLR2B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5431 |
|---|---|
| Nucleotide Accession | NP_000929.1 |
Application
POLR2B Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2B antibody detected the POLR2B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
