Application
POLR2F Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2F antibody detected the POLR2F protein (arrow).| Product Name | POLR2F Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2F; POLRF; DNA-directed RNA polymerases I, II, and III subunit RPABC2; DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; RPABC14.4; RPB6 homolog; RPC15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | POLRF |
|---|---|
| Alternative Names | POLR2F POLRF DNA-directed RNA polymerases I II and III subunit RPABC2 DNA-directed RNA polymerase II subunit F DNA-directed RNA polymerases I II and III 14.4 kDa polypeptide RPABC14.4 RPB6 homolog RPC15 |
| Molecular Weight(MW) | 14478 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) |
| Tissue Specificity | This POLR2F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the Central region of human POLR2F. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5435 |
|---|---|
| Nucleotide Accession | NP_068809.1 |
Application
POLR2F Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2F antibody detected the POLR2F protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
