Application
POLR2G Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).| Product Name | POLR2G Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2G; RPB7; DNA-directed RNA polymerase II subunit RPB7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RPB7 |
|---|---|
| Alternative Names | POLR2G RPB7 DNA-directed RNA polymerase II subunit RPB7 DNA-directed RNA polymerase II subunit G RNA polymerase II 19 kDa subunit |
| Molecular Weight(MW) | 19294 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA |
| Tissue Specificity | This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the C-terminal region of human POLR2G. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5436 |
|---|---|
| Nucleotide Accession | NP_002687.1 |
Application
POLR2G Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
