Application
POLR2I Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the POLR2I antibody detected the POLR2I protein (arrow).Application
POLR2I Antibody (N-term) (AP14668a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR2I Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POLR2I Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2I; DNA-directed RNA polymerase II subunit RPB9; DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POLR2I DNA-directed RNA polymerase II subunit RPB9 DNA-directed RNA polymerase II subunit I RNA polymerase II 14.5 kDa subunit |
|---|---|
| Molecular Weight(MW) | 14523 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity) |
| Tissue Specificity | This POLR2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human POLR2I. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5438 |
|---|---|
| Nucleotide Accession | NP_006224.1 |
Application
POLR2I Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the POLR2I antibody detected the POLR2I protein (arrow).
Application
POLR2I Antibody (N-term) (AP14668a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR2I Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
