Application
POLR2J Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2J antibody detected the POLR2J protein (arrow).Application
POLR2J Antibody (C-term) (AP14259b)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR2J Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | POLR2J Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2J; POLR2J1; DNA-directed RNA polymerase II subunit RPB11-a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | POLR2J1 |
|---|---|
| Alternative Names | POLR2J POLR2J1 DNA-directed RNA polymerase II subunit RPB11-a DNA-directed RNA polymerase II subunit J-1 RNA polymerase II 13.3 kDa subunit |
| Molecular Weight(MW) | 13293 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity) |
| Tissue Specificity | Ubiquitously expressed. High expression was found in heart and skeletal muscle |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5439 |
|---|---|
| Nucleotide Accession | NP_006225.1 |
Application
POLR2J Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2J antibody detected the POLR2J protein (arrow).
Application
POLR2J Antibody (C-term) (AP14259b)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR2J Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
